Role of mp 170 Seprase in Breast Cancer.
Abstract
Seprase/FAPalpha, a cell surface serine-type gelatinase with Mr 170, is thought to be involved in tumor invasion and metastasis. However, I have shown previously that overexpression of Seprase/FAPalpha in human SW-13 and MCF-7 epithelial carcinoma cells, has no effect on their Matrigel invasion, matrix adhesion and proliferation properties. Mammalian, bacteria and baculovirus expression systems have all been used to express and purify the molecule for further characterization, however, with little success. Rihozyme constructs targeting Seprase/FAPalpha were made, and despite their in vitro activities, none of which was effective in depleting endogenous levels of Seprase/FAPalpha in 1205LU and MDA-MB-436 cells. During the course of this project, a novel secreted fibroblast growth factor binding protein (FGF-BP2) was also characterized. I have demonstrated that FGF-BP2 has tumor promoting activity in both in vitro and in vivo. Whereas, FGF-BP2 is absent in normal breast tissues, abundant messages were induced in some breast tumor tissues, and most melanoma samples. Thus, in contrast to the apparent bystander role of Seprase/FAPalpha, preliminary evidences suggest that FGF-BP2 may play a role in cancer growth and metastasis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA374168
Entities
People
- Quang Nguyen
Organizations
- Georgetown University