X-Ray Crystallographic Studies on Acetylcholinesterase and Related Enzymes.

Abstract

Work on Torpedo acetylcholinesterase (TcAChE) involving two principal topics: A) Preparation and characterization of aged' and non-aged' organophosphoryl (OP) conjugates. B) Use of time-resolved crystallography in order to understand the mechanism of action of acetylcholinesterase (AChE), as well as the traffic of substrate and products through the active-site gorge. Structural studies on other cholinesterases (ChEs). Homology model building and detailed structural comparison of human, bovine fetal serum, snake venom and insect AChE, and of horse and human serum butyrylcholinesterase (BChE). Structural studies on human paraoxonase. The present report summanzes our principal achievements during the second year of the project. We present progress primarily with respect to Topics lA, 2 and 4, as well as in additional areas related to the long-term aims of our research program, and also a study of specific chemical damage to TcAChE produced by ionizing radiation.

Open PDF

Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1999
Accession Number
ADA375104

Entities

People

  • I. Silman
  • Joel L. Sussman

Organizations

  • Weizmann Institute of Science

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Cell Physiological Processes
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Enzyme Inhibitors
  • Fish
  • Medical Personnel
  • Organic Chemistry

Fields of Study

  • Chemistry

Readers

  • Neurotoxicology
  • Systems Analysis and Design