Cripto-1 in Mammary Gland Development and Carcinogenesis

Abstract

The purpose of this study is to evaiflate the role of the growth factor Cripto-1 (CR-i) in mammary development and carcinogenesis. During the second year of this grant, we discovered that alteration of CR-i levels affects colony formation of +SA cells. Two additional CR-i-specific ribozymes were designed and tested in vitro. Due to problems with the retrovfral vectors, we are creating adenoviral vectors for the new ribozymes and CR-i. These vectors may not be compatible with the tasks in T.O. 1, due to a lack of integration into the developing mammary epithelium. In order to perform these studies, we are aflempting to purify native CR-i for direct delivery to the developing gland.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1999
Accession Number
ADA375158

Entities

People

  • Randall L. Kintner

Organizations

  • Washington State University

Tags

DTIC Thesaurus Topics

  • Breast Cancer
  • Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemistry
  • Culture Techniques
  • Epithelial Cells
  • Epithelium
  • Genetics
  • Growth Factors
  • Mammary Glands
  • Molecules
  • Neoplasms
  • Proteins
  • Tissues
  • Tumor Cell Line

Readers

  • Molecular Genetics
  • Oncology (Cancer Research).