Effects of Environmental Estrogen on Apoptosis in Normal and Cancerous Breast Epithelial Cells

Abstract

MCF-7 cells used to examine the effects of estrogens and environmental estrogens on TN F-induced apoptosis. 17-p-estradiol, o,p'DDT and alachior were shown to suppress apoptosis in MCF-7 cells and increase expression of Bcl-2. These same compounds were unable to suppress TN F-induced apoptosis of MDA-MB-231 cells suggesting an ER-dependent mechanism of action. To investigate Bcl-2 in TNF resistance by environmental estrogens a Bcl-2 expression vector was transfected into MCF-7 cells and stable clones were established. These MOF-7N/SFFV-BCL-2 clones express higher levels of BCl-2 as compared to vector transfected MCF-7N/Neo cells.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1999
Accession Number
ADA375159

Entities

People

  • Matthew Burow

Organizations

  • Tulane University of Louisiana

Tags

DTIC Thesaurus Topics

  • Acids
  • Alcohols
  • Amino Acids
  • Anti-Bacterial Agents
  • Apoptosis
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Epithelial Cells
  • Hormones
  • Indicator Dyes
  • Neoplasms
  • Programmed Cell Death
  • Resistance
  • Tumor Cell Line

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.