Estrogen Nuclear Receptor Coactivators in Pathogenesis of Breast Cancer.
Abstract
A novel nuclear receptor coactivator of transcription, p300 and CBP associated factor (PCAF) has been demonstrated to be required for estrogen-, thyroid hormone-, and retinoic acid- dependent gene expression. The critical role of the intrinsic acetyltransferase enzymatic activity of PCAF in hormone regulated transcription provides a link between the function of this coactivator and steroid dependent regulation of cell growth in pathogenesis of breast cancer. It has been demonstrated that the enzymatic activity of PCAF protein is blocked by the adenoviral gene product, ElA, the well-known inhibitor of transcription. To examine a biological function of PCAF, a gene "knock in" technology has been employed in order to generate a mutant mice expressing PCAFDelta HAT harboring two amino acids substitution mutation which eliminates the enzymatic activity. Analysis of PCAF expression in mouse embryo has shown ubiquitous expression pattern suggesting general importance of the gene for a cellular function.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA375243
Entities
People
- Edward Korzus
Organizations
- University of California, San Diego