Malaria Genome Sequencing Project.

Abstract

The objectives of this 5-year Cooperative Agreement between TIGR and the Malaria Program, NMRC, were to: Specific Aim 1, sequence 3.5 Mb of P. falciparum genomic DNA; Specific Aim 2, annotate the sequence; Specific Aim 3, release the information to the scientific community. To date, we have published the first complete sequence of a malarial chromosome (chromosome 2 4 ), completed the random phase sequencing of 3 other large chromosomes totaling 7.2 Mb, and have initiated functional genomics studies using glass slide micorarrays to characterize the expression of chromosome 2, 3, and 14 genes throughout the erythrocytic cycle. We have also collaborated in the construction of a two-enzyme optical restriction map of the entire P. falciparum genome 7 , and are continuing to further develop the GlimmerM gene finding software developed in year 1. In addition, we have begun small scale sequencing of the rodent malaria P. yoelii and are collaborating in the sequencing of part of a P. vi vax chromosome. Discussions with the Malaria Program, NMRC aimed at development of a program to use genomics and functional genomics to accelerate vaccine research are in progress.

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Document Details

Document Type
Technical Report
Publication Date
Jan 01, 2000
Accession Number
ADA376886

Entities

People

  • Malcolm J. Gardner

Organizations

  • J. Craig Venter Institute

Tags

Communities of Interest

  • Advanced Electronics
  • Autonomy
  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Bacteria
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Chromosomes
  • Computational Science
  • Databases
  • Dna Microarrays
  • Fungi
  • Genetic Structures
  • Genetics
  • Lymphocytes
  • Malaria
  • Microbial Genome
  • Microbiology
  • Proteins
  • Vaccines

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Molecular and genetic basis of cancer.
  • Parasitology and Pharmacology of Malaria.

Technology Areas

  • Biotechnology