Prostate Carcinoma Metastasis Tracked with Histochemical Marker Genes.
Abstract
This project examines mechanisms by which human prostate carcinoma (PCA) cells undergo metastasis in an athymic nude mouse (male) model system since little attention has been devoted to these events for PCA. This includes PCA CWR22, CWR22R, and CWR21 xenografts adapted to tissue culture. To track tumor cells at the single-cell level and quantitatively, histochemical marker genes will be transfected for resolution as blue-, red-, or black-staining cells. Tissue culture lines of CWR21 have now been isolated for the first time--CWR21A and B; these are highly metastatic to several organ systems. Specific aim I examines the organ specificity of metastatic spread (particularly to bone and liver which escape detection in most animal models). LacZ-tagged CWR22R cells have been isolated and s.c. injected. We routinely observe micrometastasis to lung, liver, and bone (in select cases to brain). Specific aim II will test any significance for androgen-dependence or independence in metastatic spread, particularly for organ specificity. Androgen-relatedness of metastasis of CWR21A, B and lacZ-CWR22R cells is being evaluated. Specific aim III will evaluate possible interclonal cooperativity by injecting two different PCA cell types tagged with different marker genes; PAP-tagged CWR21A, B and CWR22.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA377261
Entities
People
- Llyod A. Culp
Organizations
- Case Western Reserve University