Cell Cycle Analysis of the BRCA1 Gene Product
Abstract
During the past 12 months, our laboratory has made significant progress in the tasks of this application related to the discovery of specific BRCA1 interacting proteins. We have discovered that BRCA2 is a major BRCA1 binding protein and, based upon the data, suggested that both proteins operate together in a signaling pathway dedicated to DNA damage/genome integrity control. In addition, we have accumulated data which suggest that the function of this pathway is linked to the suppression of breast and ovarian cancer. In more recent experiments, we have discovered a novel protein (p130) that specifically interacts, in vivo, with the BRCT domains of BRCA1. We have cloned this polypeptide and deciphered its amino acid sequence, which indicates that its product is a new member of a family of DNA-dependent helicases. In this regard, it is clearly related the ERCC4 (XP-D) class of nucleotide excision repair proteins. We are now engaged in experiments aimed at deciphering the biochemical and,ideally, the biological implications of this interaction, which can now be readily detected in untransfected human cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA377810
Entities
People
- David M. Livingston
- Dennis Lynch
Organizations
- Dana–Farber Cancer Institute