Isolation of Novel Prostate Cancer Tumor Suppressor Genes in African American and Caucasian Men thru Laser Microdissection and Representation Difference Analysis.
Abstract
Significant progress has been made for all three specific aims. Specific Aim 1: Obtaining metastatic prostate cancer DNA of quality sufficient for Representational Difference Analysis (RDA), has been achieved using alternate means. Specific Aim 2: Employ RDA to identify DNA regions which have been homozygously deleted, has been achieved with identification of a novel region of homozygous deletion on chromosome 12p (Cancer Research, 58, 5652-5655, 1998). Specific Aim 3: To prioritize regions of homozygous deletion based on frequency of deletion of this region in metastatic tumors, and to identify candidate genes within these regions, has been achieved, with previously unidentified deletion of the identified chromosome 12p region in 50% of metastatic prostate cancers studied (Genes, Chromosomes, and Cancer 25:270-276, 1999) . Examination of genes within this region suggests that p27 or TEL to be likely candidates. These results validate this approach and additional studies are underway to identify additional genomic regions of interest in metastatic prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA378061
Entities
People
- G. S. Bova
Organizations
- Johns Hopkins University