Prevention of Breast Cancer Cell Transformation by Blockade of the AP-1 Transcription Factor
Abstract
During the second year of the funding period we have investigated the affect of AF-1 blockade on the growth of normal, immortal, and fully malignant breast cells. These studies have demonstrated that the growth of normal and immortal cells is suppressed by AP-1 blockade, while the growth of breast cancer cells is not suppressed. We have also generated breast cancer cell lines which express the AP-1 inhibitior, TAM67, under inducible conditions. The growth of these cancer cells was shown to be insensitive to AP-1 blockade, consistent with our previous results. These cell lines are necessary reagents to address studies proposed in specific aim 2 and specific aim 3 for months 25-48. In the following years of funding we will utilize these reagents to investigate the role of AP-1 transcriptional activity in growth factor-dependent proliferation and oncogene-induced transformation of breast cells. These studies have demonstrated an involvement of AP-1 transcription complexes in regulating human breast cell proliferation at different stages of the transformation process. The results from these studies and will provide the foundation for future efforts to develop agents which interfere with AP-1 signaling pathways. Such agents may be useful chemopreventive agents to block breast carcinogenesis.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1998
- Accession Number
- ADA378104
Entities
People
- Powel Brown
Organizations
- University of Texas Health Science Center at San Antonio