Cell Signalling by a Novel SH2 Domain Protein that is Overexpressed with Her2 in Breast Cancer
Abstract
The goal of this project is to determine the role of the Src homology 2 domain protein, Grb7, in receptor tyrosine kinase signal transduction. Grb7 is overexpressed in approximately twenty percent of breast cancers coordinately with the HER2 receptor tyrosine kinase. Grb7 binds to HER2 but the role of Grb7 in HER2 signaling is unknown. Our previous attempts to analyze Grb7 function by overexpressing the protein have not been successful. We are unable to detect a phenotype in breast or kidney epithelial cells that overexpress Grb7. We are now generating Grb7 deficient mice as another approach to analyze Grb7 function. We have made good progress in this regard having generated chimeric males containing cells in which one copy of Grb7 has been eliminated. We are currently mating these chimeric males and are generating mice with one copy of Grb7 deleted and mice that do not express Grb7. The mice that do not express Grb7 should provide insight in the role of Grb7 in the normal development of the breast and other organs. They will also be useful in analyzing the effects of decreased Grb7 expression on HER2 induced breast cancer in mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1998
- Accession Number
- ADA378116
Entities
People
- Benjamin L. Margolis
Organizations
- NYU Langone Health