Molecular Genetics of Breast Neoplasia.
Abstract
The overall objectives of this grant were a) to develop new multiparametric fluorescence in situ hybridization (M-FISH) techniques for the analysis of chromosomal abnormalities and genetic alterations in breast cells and tissues and b) to identify genes in the p14-21 region of chromosome 3 that may be implicated in the pathophysiology of breast neoplasia. We were successful in developing and validating an M-FISH karyotyping procedure in which each chromosome is uniquely identified by combinatorially fluor-labeled chromosome painting probes. M-FISH was also carried out with band-specific probes as well as genomic DNA clones specific for chromosomal regions implicated in breast cancer. Unfortunately, the development of computer algorithms for 3-D image deconvolution were extremely difficult and time-consuming, thus M-FISH on surgical sections or archival specimens could not be done. We demonstrated that mRNA transcripts from the fragile histidine triad (FHIT) gene, that maps within the 3p14-21 interval was aberrant in approximately 40% of the primary breast carcinomas examined,, indicating that the FHIT gene may be involved in breast cancer progression. Finally, we have shown that comparative genome hybridization (CGH) of needle aspirates from breast tumors detect the same chromosomal changes as found in surgical specimens, facilitating evaluation of tumors prior to surgery.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1998
- Accession Number
- ADA378177
Entities
People
- David C. Ward
Organizations
- Yale University