Vascular Endothelial Growth Factor and Receptors in Breast Cancer

Abstract

Experiments completed in the past 6 months (Oct. 1, 1998 - March 30, 1999) were designed to characterize the localization at light and electron microscopic level of the Vascular Endothelial Growth Factor receptor Flk-1 on normal and breast cancer tissues. A novel polyclonal antibody (72 Ab) has been raised against Flk-1, affinity purified and used for these studies. Our findings show that the 72 Ab, while useful for detection by immunofluorescence of the receptor in cultured cells overexpressing Flk-1, did not give a consistent signal on samples from murine kidney and breast tumors grown on nude mice.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1999
Accession Number
ADA378724

Entities

People

  • George E. Palade

Organizations

  • University of California, San Diego

Tags

DTIC Thesaurus Topics

  • Alcohols
  • Antibodies
  • Biological Factors
  • Blood
  • Breast Cancer
  • Cancer
  • Cells
  • Chemical Synthesis
  • Chemistry
  • Endothelial Cells
  • Growth Factors
  • Kidneys
  • Neoplasms
  • Nervous System Neoplasms
  • Proteins
  • Rodents
  • United States

Fields of Study

  • Biology

Readers

  • Immunology
  • Oncology (Cancer Research).

Technology Areas

  • Microelectronics