X-Ray Crystallography of Botulinum Neurotoxins

Abstract

Botulinum Neurotoxin type E was purchased, and the protein was crystallized. The best diffracting crystals were obtained when the toxin was crystallized in the presence of a peptide from SNAP-25. An isomorphous tungsten derivative has been prepared and data have been collected to 3.2A resolution. Three heavy atom sites were identified and used in phasing by the single isomorphous replacement with anomalous scattering method. The initial low resolution map clearly shows the protein envelop and also some protein features. Two additional heavy atom derivative candidates have been prepared.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 2000
Accession Number
ADA378753

Entities

People

  • Martin Sax
  • S. Swaminathan
  • W. Furey

Organizations

  • Veterans Administration Medical Center

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Crystal Structure
  • Crystallography
  • Crystals
  • Data Sets
  • Department Of Veterans Affairs
  • Diffraction
  • Laboratory Animals
  • Materials
  • Neurotoxins
  • Recombinant Dna
  • Scattering
  • Toxins
  • Tungsten
  • X Rays
  • X-Ray Crystallography
  • X-Ray Diffraction

Fields of Study

  • Chemistry

Readers

  • Materials Science and Engineering.
  • Microbial Pathology
  • Systems Analysis and Design