Development of a Reproductive Toxicity Test Using Xenopus.

Abstract

Current reproductive toxicity tests may be missing significant toxicity by not examining the gametes of both male and female organisms. The purpose of the present study was to develop and validate a reproductive toxicity test using Xenopus laevis which measures effects on gametogenesis, reproductive performance, and development fitness of the progeny. Sexually mature female and male Xenopus laevis were exposed to varying concentrations of cadmium chloride (Cd), boric acid (B), or ethylene glycol monomethyl ether (EGME), in order to examine the effects on gametogenesis and reproductive outcome. Frogs were exposed for a period of 30 days and then either bred to untreated males/females or their gonads were examined to determine effect on gametogenesis. Results from these studies indicated that each toxicant was capable of inducing adverse reproductive effects including gametogenesis, reproductive performance and development effects in a concentration-dependent manner. Preliminary evaluation of the protocol for reproductive assessment appear favorable.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 2000
Accession Number
ADA379162

Entities

People

  • Douglas J. Fort
  • John A. Bantle

Organizations

  • Oklahoma State University–Stillwater

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Boric Acids
  • Cadmium Compounds
  • Cell Physiological Processes
  • Cells
  • Cellular Structures
  • Chemical Analysis
  • Chemistry
  • Ethylene Glycol
  • Fish
  • Intercellular Junctions
  • Mass Spectrometry
  • Sex Glands
  • Statistical Analysis
  • Test And Evaluation
  • Toxicity
  • Wildlife

Readers

  • Aquatic Ecology