Single-Shot Fluorescence Spectra of Individual Micrometer-Sized Bioaerosols Illuminated by a 351- or a 266-nm Ultraviolet Laser
Abstract
Reproducible fluorescence spectra of individual 2 to 5 micrometers diameter biological aerosol particles excited with a single shot from a Q-switched laser (266 or 351 nm) have been obtained with highly improved signal-to- noise ratios. Critical to the advance are crossed diode-laser trigger beams, which precisely define the sample volume, and a reflecting objective, which minimizes chromatic aberration and has a large N.A. for collecting fluorescence. Several allergens (red oak, meadow oat pollen, paper mulberry pollen, and puffball spores) have different fluorescence spectra. Bacillus subtilis fluorescence spectrum deteriorates at high 266-nm incident intensity. Dry riboflavin particles illuminated with a 351-nm light exhibit a new 420-nm fluorescence peak that grows nonlinearly with laser pulse energy.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 15, 1999
- Accession Number
- ADA379175
Entities
People
- Jerold Bottiger
- Richard K. Chang
- Ronald G. Pinnick
- Stanley Niles
- Stephen Holler
- Steven C. Hill
- Yong-Le Pan
Organizations
- Yale University