Mosaic Landscape Phage as a New Class of Detector

Abstract

Detection of single molecules or particles of biological or organic agents requires a ligand, which could be immobilized to a chip of analytical platform and should provide strong specific binding of the agent and generation of a detectable signal. We suggested new ligands-landscape phages selected from landscape libraries or mosaic landscape libraries. By testing landscape libraries with a panel of representative antigens (streptavidin from the bacterium Streptomyces avidinii, avidin from chicken egg white, and b-galactosidase from Escherichia coli) we obtained a proof of conception of using landscape phage as a new type of ligand for detector platforms. A library of organic landscapes is a rich source of substitute antibodies-filaments that can bind protein and glycoprotein antigens with nanomolar affinities and high specificity.

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Document Details

Document Type
Technical Report
Publication Date
Apr 19, 2000
Accession Number
ADA379225

Entities

People

  • George P. Smith
  • Valery A. Petrenko

Organizations

  • University of Missouri

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Amino Acids
  • Antibodies
  • Bacterial Proteins
  • Biomedical And Dental Materials
  • Chemical Compounds
  • Chemistry
  • Chloroform
  • Clones
  • Demographic Cohorts
  • Detectors
  • Engineering
  • Escherichia
  • Escherichia Coli
  • Molecules
  • Polymers
  • Proteins

Readers

  • Molecular Genetics
  • Wetland-Land-Environmental Management.