Role of Seprase in Breast Cancer
Abstract
This report documents progress made in the third year of DAMDI7-96-1-6097 "Role of seprase in breast cancer invasion". The goals are to clone a full length cDNA encoding seprase and then produce cells that overexpress seprase to investigate its role in tumor cell invasion. A chicken embryo seprase cDNA has been used to produce a seprase-calmodulin binding protein fusion protein that will be used to produce new antibodies to seprase. Full-length (2.4 kb) and truncated (1.1 kb) seprase cDNAs have been cloned from MDA-MB-436 human breast cancer cells and characterized. Seprase over-expression by breast cancer cells has been directly demonstrated biochemically and with seprase-specific cDNA probes. Moreover, we have demonstrated that MDA- MB-436 cells express active seprase. MDA-MB-436 cells that are down-regulated with respect to cell- surface seprase expression have been produced by transfection with seprase DNA expressed in the antisense orientation. Two papers describing new results regarding this project were published this year. The research progress is on time with respect to the original statement of work and we expect successful completion of the proposed research within the 4 year project period.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA379335
Entities
People
- Thomas J. Kelly Jr.
Organizations
- University of Arkansas at Little Rock