Genotoxicity Testing in Sediments: Progress in Developing a Transgenic Polychaete Model
Abstract
This technical note describes progress made during calendar year 1999 in the development at the U.S. Army Engineer Research and Development Center (ERDC) of a transgenic marine polychaete worm for testing genotoxicity of sediments. In keeping with the intent of previous communications, this technical note contains protocols for laboratory procedures to aid other researchers who may be similarly engaged in the development of transgenic invertebrate models for research or testing purposes. Protocols are provided for cryopreservation of polychaete sperm and for transgene insertion using electroporation combined with osmotic shock.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2000
- Accession Number
- ADA379487
Entities
People
- Laura S. Inouye
- Victor A. Mcfarland