Aerobic Degradation of Dinitrotoluenes and Pathway for Bacterial Degradation of 2,6- Dinitrotoluene.
Abstract
An oxidative pathway for the mineralization of 2,4-dinitrotoluene (2,4-DNT) by Burkhoderia sp. strain DNT has been reported previously. We report here the isolation of additional strains with the ability to mineralize dinitrotoluene (2,6-DNT) by a different pathway. Burkizodena cepacia strain JS85O and Hydrogenephaga palleronii strain JS863 grew on 2,6-DNT as the sole source of carbon and nitrogen. The initial steps in the pathway metabolites through mass spectroscopy and nuclear magnetic resonance. 2,6- DNT was converted to 3-methyl-4-nitrocatechol by a dioxygenation reaction accompanied by the release of nitrite. 3-Methyl-4- nitrocatechol was the substrate for extradiol ring cleavage yielding 2-hydroxy-5-nitro-6-oxohepta-2,4-dienoic acid, which was converted to 2-hydroxy-5 -nitropenta-2,4-dienoic acid.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2000
- Accession Number
- ADA379526
Entities
People
- George C. Paoli
- Jim C. Spain
- Shirley F. Nishino
Organizations
- Air Force Research Laboratory