Evaluation of Drug and Vaccine Candidates in the Human Malaria/Aotus Monkey Model
Abstract
A C2A clone of a Mefloquine resistant Plasmodium faiciparum strain was successfully adapted to splenectomized and intact Aotus. Chioroquine resistance reversal was achieved in 3/3 Aotus infected with the AMRU-1 strain of Plasmodium vivax by using chloroquine at 1Omg/kg and prochiorperazine at 20 mg/kg in combination. Artelinic Acid (WR255663AK;BM041 31) when given orally at 2 mg/kg x three days suppressed infections of the AMRU-1 (CQR) but cleared SAL-1 strains of P. vivax in Aotus monkeys. Artelinic Acid (WR255663AK;BM04131) administered orally at 2-24 mg/kg x three days was effective against infections of P. falciparum FVO strain in Aotus monkeys. Orally or intravenously administered falcipain (APC33l 7) was ineffective against infections of P. falciparum FVO. Artelinic Acid and Artesunate were effective against infections with P. falciparum FVO in Aotus monkeys. Passive transfer of anti-EBA-175 Region II protein monoclonal antibodies was not effective at controlling parasitemia in Aotus monkeys infected with P. falciparum. Immunization with a plasmid encoding region ll of EBA-1 75 followed with by a EBA-175 recombinant protein boost partially protected Aotus monkeys against P. falciparum malaria. Topical ocular administration of a plasmid DNA vaccine encoding an AMA-1 P. falciparum blood stage antigen did not induce an immune response in Aotus monkeys.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 2000
- Accession Number
- ADA379590
Entities
People
- Nicanor Obaldia