Cloning of Tumor Suppressor Genes in Prostate Cancer by a Novel Tumor Reversion Method
Abstract
We have proposed a novel approach to the cloning of tumor suppressor genes in prostate cancer. We have developed methods for the transfer of large pieces of human DNA cloned into bacterial artificial chromosomes (BACs) into human prostate cancer cell lines. For this purpose, we target the BACs with drug resistance and other markers that will allow them to be stably transferred into the cell lines, and then transfer them into several different prostate cancer cell lines. For the next stage of this project, we plan to use several different assays to test the ability of the transferred human DNA to revert (render less tumorigenic) the neoplastic phenotype of the cancer cell lines. These assays will include morphological changes in the cells, doubling time, growth in soft agar, and tumorigenicity in immunodeficient mice. The assays will be used to determine if a particular BAC contains a gene that reverts the transformed phenotype of the cell line, and therefore contains a human tumor suppressor gene. BACs that revert cell lines in this assay will then be fragmented or subdivided, and the subclones tested in our assays. In this way, the tumor suppressor gene on the BAC will be localized precisely.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA380214
Entities
People
- Graeme B. Bolger
Organizations
- University of Utah