Novel Technology for Cloning Prostate Cancer Cell Markers
Abstract
The purpose of the research is to clone, isolate and characterize protein markers that are specifically expressed by advanced stage, androgen-independent prostate cancers. Two types of markers with this specificity are expected to be identified: (1) proteins secreted specifically by advanced stage prostate cancer cells, potentially useful in serum-based assays designed to specifically detect this class of tumors; and (2) markers expressed specifically on the surface of these cells , potentially useful as specific targets for clinical treatment of androgen-independent prostate cancer. Progress to date has involved: (1) preparation of membrane fraction mRNA's from four human cell lines representing four stages of prostate cancer, and use of these mRNA's to prepare cDNA libraries; (2) isolation of proteins from membrane fractions of two of these cell lines, and initiation of a collaboration to employ the techniques of Proteomics to differentially analyze these protein populations. We can now apply the new DNA microarray technology to the cDNA libraries described in (1) to identify clones possessing the characteristics described above. In addition, we are now employing the proteins described in (2) above for differential screening via two-dimensional gel analysis, to directly identify cell surface proteins specifically expressed by advanced stage prostate cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA380284
Entities
People
- F. C. Bancroft
Organizations
- Mount Sinai Hospital