Differentially Expressed Genes in Human Prostatic Carcinoma
Abstract
In spite of the fact that prostate cancer has become a significant health burden, its molecular determinants are poorly understood. We have proposed to identify the genes that are differentially expressed in prostate cancer. One type of such gene is tumor suppressor gene. During the first year of this project, we applied the methods of tissue microdissection and deletion mapping to localize tumor suppressor genes on human chromosome 13. We also used PCR-SSCP and direct sequencing methods to analyze candidate genes for their involvement in prostate cancer. We identified three regions of chromosome 13 for harboring tumor suppressor gene, i.e., 13q14, 13q21, and 13q33. The ERCC5/XPG DNA repair gene at 13q33 was not involved in prostate cancer, neither was the RBl gene at 13q14. Therefore, each of the three tumor suppressor genes remains to be identified. More interestingly, alterations of 13q14 and 13q21 appeared to be specifically responsible for the aggressive behavior of prostate cancer. Currently, we are performing additional experiments to narrow down the deletion regions that harbor the tumor suppressor genes and started searching for the genes. Identification of these genes will provide targets for improving the diagnosis and therapy of prostate cancer.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA380286
Entities
People
- Jin-Tang Dong
Organizations
- University of Virginia