Vacuolar-Type H+-ATPases in Human Breast Cancer
Abstract
Vacuolar-type ATPases are heteromultimeric proteins found in all eukaryotic cell types. The ll5kD subunit is membrane-bound component of unknown function. The subunit has been cloned, and PCR products coding for non-transmembrane domains generated for ligation into a fusion protein expression vector and subsequent fusion protein and antibody production. Agavose gel electrophoresis of post-ligation DNA suggests inserts have been ligated into the expression vector, however no fusion protein expression has been successfully induced. Northern analysis of cancer cell lines expressing V-ATPase activity grown at different pH's suggests that ll5kD subunit mRNA levels are not altered by incubation of cells at acidic pH.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA380466
Entities
People
- Charles Fehr
- R. G. Gillies
Organizations
- University of Arizona