Biotherapy of Breast Cancer With EGF-Genistein

Abstract

Our proposed research plan involves laboratory studies using a SCID mouse model of human metastatic breast cancer, as well as in vitro MTT(3- 4,5- DIMETHYLTHIAZOL-2-YL -2,5-diphenyl tetrazolium bromide) and colony assays, using established breast cancer cell lines, to examine the potency and toxicity of various EGE-Genistein conjugates. In an effort to generate more effective conjugates, we have employed a variety of crosslinking agents and photolysis conditions. Furthermore, we have established HPLC(high performance liquid chromatography) procedures to characterize and isolate the EGF components of the reaction mixture. We have also conjugated EGF to other small molecules which by themselves have been shown to possess anti-cancer activity. The knowledge gained from these studies is expected to lead to more effective biotherapy and combined biochemotherapy regimens for the treatment of breast cancer patients.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1999
Accession Number
ADA380880

Entities

People

  • Roland Gunther

Organizations

  • University of Minnesota

Tags

DTIC Thesaurus Topics

  • Bone Marrow
  • Breast Cancer
  • Cells
  • Cellular Structures
  • Chemical Synthesis
  • Chemistry
  • Chromatography
  • Endocrine Glands
  • Fish
  • Large Intestine
  • Liquid Chromatography
  • Lymphatic System
  • Mammary Glands
  • Medical Personnel
  • Neoplasms
  • Peripheral Nervous System
  • Small Molecules

Readers

  • Computational Linguistics
  • Oncology (Cancer Research).
  • Polymer Science and Technology