Analysis of HRAD1, a Human G2 Checkpoint Control Gene

Abstract

The purpose of this research is to determine the role of the hRAD1 gene in breast cancer. The research includes the generation and analysis of cell lines lacking hRAD1. The analysis includes an assessment of checkpoint proficiency and genomic instability in these cells. The research also includes a biochemical characterization of the hRAD1 protein, including its subcellular localization and protein-protein interactions with other checkpoint proteins. These other checkpoint proteins include BRCA1, hHUSl, and hRAD9. hRAD1 does not interact with BRCA1 but does form a complex with hHUS1 and hRAD9.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1999
Accession Number
ADA381034

Entities

People

  • Blair Besley
  • Scott Davey

Organizations

  • Queen's University

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Eukaryotes
  • Fungi
  • Gamma Rays
  • Genetic Structures
  • Genomic Instability
  • Instability
  • Ionizing Radiation
  • Materials
  • Neoplasms
  • Protein-Protein Interactions
  • Proteins

Readers

  • Instructional Design and Training Evaluation.
  • Molecular Biology and Genetics