Diagnosis and Dosimetry of Exposure to Sulfur Mustard: Development of Standard Operating Procedures; Further Exploratory Research on Protein Adducts
Abstract
In continuation of previous grants, Standard Operating Procedures (SOPs) have been developed for retrospective detection of exposure to sulfur mustard, i.e., an immunoslotblot assay for DNA adducts in blood and skin, and the modified Edman procedure for determination of adducts to the N-terminal valine in hemoglobin. Both SOPs were validated (day-to-day variability, inter- and intraindividual variation) and could be properly set up and carried out at another institute (MRICD) within one working day. In vivo persistence studies showed that in hairless guinea pigs the DNA adduct in blood is still detectable at 14 days after i.v. administration of sulfur mustard (0.5 LD5O), while the adduct to N-terminal valine of hemoglobin can still be analyzed after 56 days. In skin the DNA adduct level is only slowly decreasing during the first 3 days and is marginal at 17 days after skin exposure of the hairless guinea pig. In marmosets the DNA adduct is detectable in blood during 7 days after i.v. administration of sulfur mustard (4.1 mg/kg). The adduct to N-terminal valine of hemoglobin is still detectable 94 days after exposure. Furthermore, exploratory research has been performed aiming at the development of a fieldable immunochemical assay for sulfur mustard adducts with hemoglobin, albumin and keratin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 2000
- Accession Number
- ADA381035
Entities
People
- Daan Noort
- Govert P. Van Der Schans
- Hendrik P. Benschop
- Leo P. De Jong
Organizations
- Prins Maurits Laboratorium TNO