Enhancement of Breast Cancer Therapy by 6 -Aminonicotinamide
Abstract
6-Aminonicotinamide (6AN) has been shown to enhance the effect of radiation both in vivo and in vitro in a murine tumor models. This study was undertaken to determine whether enhanced efficacy of radiation, adriamycin and taxol could be obtained by pretreating with 6AN. MCF-7 cells were grown on beads and perfused in the NMR magnet. P31 NMR spectra were obtained on cells at 4.7T (81.03 MHz) which were treated with 6AN or control. Treatment with 6AN caused a new peak to be detected which has been previously assigned to 6-phosphogluconate. In addition, a decrease in phosphocreatine was noted. These results were analogous to results found with murine RIF-1 tumor cells. Surviving fraction studies were subsequently done. Cells were perfused for 4 hours with 6AN, the 6AN washed out and the cells incubated for 3 hours before being treated with radiation, adriamycin or taxol. Enhanced efficacy to radiation (2 Gy dose) and adriamycin (10 nM) was noted. 6AN inhibited the effect of Taxol. In vivo studies have been started and it appears that the maximum tolerated dose for 6AN will be between 10 and 12 mg/kg.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA381102
Entities
People
- Jason A Koutcher
Organizations
- Memorial Sloan Kettering Cancer Center