Development of PEA3 as a Potential Gene Therapy Agent for Breast Cancer

Abstract

HER-2/neu overexpression plays an important role for cancer development. To suppress cell transformation mediated by HER-2/neu overexpression, we identified a DNA- binding protein PEA3 of the Ets gene family which specifically targeted a DNA sequence on the HER2/neu promoter and downregulated the promoter activity. Expression of PEA3 resulted in preferential inhibition of cell growth and tumor development of HER- 2/neu-overexpressing cancer cells. A competition model to elucidate the molecular mechanism for the transcription inhibition of Her-2/neu by PEA3 was proposed. PEA3 directly binds to the HER-2/neu promoter and competition of PEA3 with other Ets transactivating factor(s) for a positive regulatory motif on the Her-2/neu promoter may acount for the downregulation of Her-2/neu transcription by PEA3.

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Document Details

Document Type
Technical Report
Publication Date
Oct 01, 1999
Accession Number
ADA381107

Entities

People

  • Zhenming Yu

Organizations

  • The University of Texas MD Anderson Cancer Center

Tags

DTIC Thesaurus Topics

  • Biomedical And Dental Materials
  • Breast Cancer
  • Carrier Proteins
  • Cell Line
  • Cells
  • Chemistry
  • Competition
  • Culture Techniques
  • Diseases And Disorders
  • Dna-Binding Proteins
  • Gene Therapy
  • Medical Personnel
  • Molecules
  • Neoplasms
  • Proteins
  • Therapy
  • Virotherapy

Fields of Study

  • Biology

Readers

  • Molecular Genetics
  • Naval Architecture and Marine Engineering.
  • Oncology

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech