Characterization of Two Proteins Which Interact With the BRCA1 Gene

Abstract

We have made extensive progress in the past year to support our analysis of the BAP1 - BRCA1 interaction. We have published the co-localization and cell-cycle patterns of internuclear distribution for the two proteins. In addition, we have shown that BAP-1 is a bonafide tumor suppressor gene which is able to inhibit the malignant growth characteristics of cell lines when transfected back into the BAP1 null cells. This activity is dependent upon enzymatic function of the ubiquitin hydrolysis domain. In exciting preliminary studies we have shown that BAP1, like BRCAl, has a direct role in transcription-coupled DNA repair. The work accomplished conforms to the original SOW and has shed new light on the role of BRCA1 as a tumor suppressor in early onset familial breast-ovarian cancer families.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1999
Accession Number
ADA381176

Entities

People

  • Frank J. Rauscher

Organizations

  • University of Pennsylvania

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biomedical And Dental Materials
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Cytoskeleton
  • Epithelial Cells
  • Escherichia Coli
  • Genetics
  • Immediate-Early Proteins
  • Immune Serums
  • Intranuclear Space
  • Lymphocytes
  • Neoplasms
  • Polymeric Films
  • Proteins

Readers

  • Analytical Chemistry
  • Molecular Biology and Genetics
  • Molecular and genetic basis of cancer.

Technology Areas

  • Biotechnology