Function of Maximal Microvessel Density in Breast Tumor Metastasis
Abstract
Maximum microvessel density (MMVD) in primary tumors is a sensitive prognostic indicator for many solid tumors, including breast. These data imply functional significance for the "hot spots" of microvessels which are quantitated in MMVD determinations. Since prognosis is determined by metastasis, functional significance of the "hot spots" in the process of metastasis is also implied. We hypothesize that the neovascular "hot spots" are the sites of metastasis and that gene expression in the tumor in the vicinity of the "hot spot" is important in the generation of the "hot spot" and in the process of metastasis. In addition to our previous result that metastasis is correlated to tumor size, we have found a relation between metastasis and MMVD. We have developed a method to make sense amplified RNA from 1 pg of RNA. Therefore we have combined amplified RNA production with the Clontech method of template switching and long-distance polymerase chain reaction (PCR). We are able to produce quantities of copy DNA which can be used for analysis of differential gene expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA381200
Entities
People
- Sandra W. Mcleskey
Organizations
- Georgetown University