Selective DNA Delivery to Breast Cancer Cells
Abstract
This grant award seeks to develop innovative new approaches that will permit selective targeting of DNA molecules to breast cancer cells. We hypothesize that one can use specific protein or peptide sequences to direct linked DNA molecules to breast cancer cells; this hypothesis is being experimentally tested. During the period covered by this annual progress report, we have shown that bacterially-derived recombinant adenovirus penton base protein (ADPB) can be used as a subviral gene delivery system, to target linked plasmid DNA molecules into cultured human cells. We have also shown that peptide phage display technology can be used to identify phage populations which can either bind to, or internalize in, breast cancer cells, and we have constructed T7 bacteriophage particles which express high affinity ligands for cellular HER-2/neu and alphaV beta3 integrin (HER-2/neu and alphaV beta3 integrin are endocytosing cell-surface receptors which are expressed on some breast cancer cells). The findings obtained to date are generally supportive of our central hypothesis, and they have also provided us with important reagents for further studies. Future experiments will address the ability of phage-selected peptides to mediate gene transfer into breast cancer cells, both in vitro and in vivo.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 2000
- Accession Number
- ADA381202
Entities
People
- Stephen Dewhurst
Organizations
- University of Rochester