The Single Prostate Cell Transcriptome as Biological Assay
Abstract
The subject of the research in this proposal is the development of methods for the examination of molecular alterations in prostate cancer at the level of single cells. The purpose of the research is to use the developed methods to identify molecular alteration in prostate cancer cells that can be used either singly or in combination to provide insights into the molecular evolution of prostate carcinogenesis, and produce a set of molecular tools capable of influencing the clinical management patients with prostate carcinoma. The scope to the research involves the construction of cDNA libraries representing the genes expressed in selected populations of normal and neoplastic prostate cancer cells followed by the construction of microarrays suitable for comprehensive gene expression studies. These arrays are then used to evaluate methods for single-cell transcriptome amplification with the aim of identifying a cohort of cellular transcripts which correlate with, or fingerprint a cellular phenotype.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA381283
Entities
People
- Peter S Nelson
Organizations
- University of Washington