Development of General Methods for Identification of Genes Regulated by Oncogenic Stimuli or DNA Damage
Abstract
The ability of cancer cells to proliferate inappropriately is due the loss of tumor suppressor genes and the gain of function of oncogenes. Two classes of tumor suppressor genes exist, mutators and growth regulators. Mutators are genes that when mutant cause an increase in the rate of genomic instability and hence the rapid accumulation of mutations of all classes. Genes of the mutator class include mis-match repair genes involved in HPCC, DNA repair genes such as X.P., cell cycle checkpoint genes such as ATM and p53. Recently two breast cancer genes BRCA1 and BRCA2 have been implicated in DNA repair and fall into this class. The second class of tumor suppressors, growth regulators, are those directly involved in regulating cell proliferation or the ability of tumor cells to survive and metastasize. Oncogenes such as myc or ras are dominant and act in opposition to tumor suppressors. Many of the genes in these groups directly or indirectly regulate transcription and the identification of target genes is important for elucidation of these regulatory pathways. In addition, the transcriptional induction of genes in response to DNA damage is a critical component of the cellular response to DNA damage and the prevention of mutagenesis. This grant proposes to identify genes induced by DNA damage and in response to oncogenic stimuli by conventional and novel methods.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA381310
Entities
People
- Stephen Elledge
Organizations
- Baylor College of Medicine