The Role of ID Proteins in Breast Cancer
Abstract
The basic helix-loop-helix transcription factors, E12 and E47 (products of the E2A gene), have been implicated to have a role as tumor suppressors. Conversely, the inhibitors of El2 and E47, the Id proteins, have been shown to stimulate cell growth, and cause T cell lymphoma when overexpressed in the T cell lineage. This project is to test the hypothesis that Id-1 overexpression in mammary glands may contribute to the development of breast cancer. In this first year of the funding period, we have established transgenic mouse lines, pYY-Idl-30 and pYY-Idl-59, in which Id1 is expressed under the control of MMTV LTR. The biological effects of Id 1 overexpression on normal mammary gland development and tumorigenesis are being investigated. We have also found that Idi is overexpressed in two of breast cancer cell lines, T47D and MDA-MB-23 1. Consequently, the transcriptional activity of E47 (or El 2) is extremely low even though the proteins are readily detectable. We are currently testing if restoration of the transcription activity of E47 could lead to growth arrest of the breast cancer cells. These studies may establish a tumor suppressing function of E2A proteins in mammary glands, and shed light on the molecular mechanisms concerning tumorigenesis in breast tissues.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1999
- Accession Number
- ADA381311
Entities
People
- Xiao-hong Sun
Organizations
- NYU Langone Health