Interactions Among Brac1, Brac2, and Components of the Recombination Machinery
Abstract
Despite the importance of BRCA1 and BRCA2 genes in breast cancer suppression, little concrete information is available concerning the functions of their encoded products. Recent studies have indicated a physical interaction of BRCA1 with the Rad5O/Mre11/NBS1 complex and of BRCA2 with Rad51, which are key components of the RAD52 protein group that mediate homologous recombination and recombinational DNA repair. These findings strongly suggest that both BRCA1 and BRCA2 proteins have a function in recombination and DNA repair. For defining the mechanism of the recombination machinery and the roles of BRCA1 and BRCA2 proteins in recombination, we have (1) purified from nuclear extracts of Raji cells the Rad5O/Mre 11/NBS1 complex and have carried out characterization of the nuclease activities of this complex, (2) purified the Rad51 protein and established in vitro systems for examining the biochemical properties of this recombinase, and (3) expressed BRCA2 domains that are known to interact with Rad51 protein. A variety of studies will be carried out to delineate the mechanism of the recombination machinery and to examine the functional and physical interactions among components of the recombination machinery, BRCA1, and BRCA2.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1999
- Accession Number
- ADA381339
Entities
People
- Patrick M. Sung
Organizations
- University of Texas Health Science Center at San Antonio