Expression of the Estrogen-Regulated pS2 Gene in MCF-7 Human Breast Cancer Cells
Abstract
In vivo footprinting was utilized to examine the estrogen-responsive pS2 gene in MCF-7 human breast cancer cells. Estrogen treatment resulted in an extensive region of protection within and adjacent to the pS2 estrogen response element (ERE). The antiestrogens 4-hydroxytamoxifen and ICI 182,780 each produced distinct protection patterns of the pS2 ERE. The TATA and CAAT sequences were protected and flanked by hypersensitive sites in the presence and absence of hormone in MCF-7 cells but not in estrogen receptor (ER)-negative MDA MB 231 cells. To aid in future identification of proteins bound to these elements, in vitro footprinting experiments were performed utilizing purified ER and nuclear extracts from estrogen-treated MCF-7 cells. Results of in vitro DNase I footprinting experiments paralleled the protection patterns of in vivo footprinting experiments. The CAAT box was minimally protected with hypersensitive sites surrounding both the TATA and CAAT boxes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA382451
Entities
People
- Lawrence N. Petz
Organizations
- University of Illinois Urbana–Champaign