Characterization of the BRCAl Protein Product
Abstract
Breast cancer is the second leading cause of cancer-related death among women in the United States. Approximately 5-10% of breast cancer cases are associated with inheritable genetic factors. Two familial breast cancer susceptibility genes, BRCAl and BRCA2, have been isolated to date, with a third gene, BRCA3, still being actively sought. Mutations in BR CAl are associated with both ovarian and breast cancer, while mutations in BRCA2 are associated with breast cancer in both women and men. It is not known why this difference occurs, but these findings may have important implications for the development of future surveillance, diagnosis, and treatment strategies of familial breast cancer. In keeping with the design of our original proposal, we have successfully generated a panel of monoclonal antibodies directed against various regions of the BRCA2 protein as a result of three independent fusions. In addition, we have cloned the full-length BRCA2 coding sequence into various mammalian expression vectors to study BRCA2 function in either transient or stable-inducible expression systems. We are now in the process of characterizing our antibodies and beginning experiments studying aspects of BRCA2's putative functions in both DNA-repair and transcriptional activation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA382528
Entities
People
- John J. Park
- Michael Press
Organizations
- University of Southern California