Developing a System for Directed Gene Introduction into Mammary Gland Via Targeted Infection of Retrovirus Receptor Transgenics
Abstract
The long term goal of this project is to develop a novel method to target infection of retroviral vectors in vivo utilizing mice expressing a retroviral receptor transgene (the Rous sarcoma virus receptor). Directed infection, and thus directed gene expression of cells expressing the viral receptor should provide a rapid and efficient method to test the mammary tumorigenic potential of genes in an animal model. Unlike the traditional method of testing gene function in transgenic mice, directed infection can be temporally controlled allowing assessment of differences in oncogenic potential at different stages of mammary gland development. Finally, multiple oncogenes can be introduced by co-infection allowing questions of synergy to be addressed. Toward this long term goal, we produced two transgenic mouse lines carrying the RSV receptor. However before characterization of receptor expression or in vivo targeting, both transgenic lines were lost. Considerable progress on development of viral vectors for use in this targeting system was achieved. Vectors and procedures for the production of high titer murine leukemia virus (RSV) pseudotypes were established. These MLV(RSV) vectors allow greater flexibility and capability compared to available RSV vectors and will be of great utility for directed infection of mammary epithelial cells in transgenic mice when the mice become available.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1998
- Accession Number
- ADA382556
Entities
People
- Paul Bates
Organizations
- University of Pennsylvania