Allosteric Modifiers of Hemoglobin: Potential Applications in Red Cell Storage and Liposome-Encepsulated Hemoglobin Development

Abstract

The overall objective of the research program is to develop allosteric modifiers of hemoglobin? that are effective in vivo. The ability of a novel agent 02-50, a trifluoromethyl substituted analog of LRl6, was found to strongly modulate the P10 value of purified hemoglobin. 02-50 displayed activity greater than that of LRl6. However, the presence of human serum albumin (HSA) strongly modulated the pharmacological properties of LRl6 and 02-50 agents. Experiments with denatured HSA also revealed that the albumin interactions with LR-16 were not specific in nature and both LR-16 and 02-50 are lipophilic and capable of diffusing from LEH particles.

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Document Details

Document Type
Technical Report
Publication Date
Nov 30, 1998
Accession Number
ADA382644

Entities

People

  • Danzhou Yang
  • Stean Kruszeweki
  • Thomas G. Burke

Organizations

  • University of Kentucky

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Albumins
  • Amino Acids
  • Blood
  • Blood Proteins
  • Chemistry
  • Fluorescence
  • Hemoglobin
  • Lipids
  • Measurement
  • Membrane Lipids
  • Molecules
  • Oxygenation
  • Partial Pressure
  • Particles
  • Phase Transformations
  • Polymers
  • Proteins

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Parasitology and Pharmacology of Malaria.