Gene Therapy Mediated Breast Cancer Immunity
Abstract
The goat of the study was to assess the efficiency of B7-1 expressing breast cancer cells as a tumor vaccine. We showed that adenovirally delivered B7-1 expression on mammary carcinoma cells did not result in tumor relection in vivo and failed to activate allogeneic T cells in vitro. We prnvided evidence that the lack of T cell stimulation by B7-1 expressing breast cancer cells was due to secretion of PGE2. POE2 is produced by the cyclooxygenase (COX) mediated oxidation of arachidonic acid. The inhibition of COX activity in B7-1 expressing MDA-MB 231 cells restored the proliferative response of T cells. To further support the finding that POE2 inhibits B7-i induced T cell responses we created B7-i positive, COX-i expressing HBL-100 breast epithelial cells and WM9 melanoma cells. T cells were stimulated to proliferate by B7-i expressing HBL-i00 cells or WM9 cells, whereas T cell proliferation was inhibited when both P7-l and COX-l were expressed. Therefore, POE2 may limit the use of breast cancer vaccines based on B7-1 expressing breast cancer cells.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1999
- Accession Number
- ADA382694
Entities
People
- Heike K. Nesbit
Organizations
- University of Pennsylvania