Role of Nuclear Matrix in Estrogen Regulated Gene Expression in Human Breast Cancer Cells
Abstract
The goal of this research is to study association of the estrogen receptor alpha (ER) with the nuclear matrix, and to identify nuclear matrix sites for ER. We constructed an ER fusion protein (GFP-ER) with a His6 tag, HA tag and the green fluorescent protein. Using fluorescent imaging techniques we studied the cellular localization of GFP-ER in the presence of various ER ligands in human breast cancer cell lines transiently transfected to express the GFP-ER protein. Striking differences in the patterns of distribution were observed. Isolation of GFP-ER associated proteins requires a large number of cell expressing GFP-ER. Therefore, a MOF7 stable cell line expressing GFP-ER under the control of an inducible promoter was constructed. Levels of GFP-ER expression can be controlled in this cell line, and this cell line has been carefully analyzed to determine the effect of GFP-ER expression on cell growth and gene expression.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1999
- Accession Number
- ADA382697
Entities
People
- Laurel T. Holth
Organizations
- University of Manitoba