ErbB2 Regulation of c-src Activity in Breast Cancer

Abstract

ErbB2 transformation of mammary epithelial cells activated c-src. C-src activation was associated with decreased phosphorylation of tyrosine 527, but not by a decrease in C-terminal-src-kinase (CSK) activity. Instead, ErbB2 appeared to associate with and activate the phosphotyrosine phosphatase SHP2. SHP2 appears to be necessary for ErbB2 to activate c-src. In additional studies, c-src activation was shown to be necessary for ErbB2-induced cell growth on soft agar and in athymic mice. These results suggest that ErbB2-mediated activation of the phosphatase SHP2 may be a critical event in tumorigenesis, possibly via the activation of c-src.

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Document Details

Document Type
Technical Report
Publication Date
Jul 01, 1999
Accession Number
ADA382731

Entities

People

  • Lewis G. Sheffield

Organizations

  • University of Wisconsin–Madison

Tags

DTIC Thesaurus Topics

  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cell Physiological Processes
  • Cells
  • Chemistry
  • Epithelial Cells
  • Kinases
  • Materials
  • Neoplasms
  • Peptides
  • Phosphorylation
  • Polyomaviridae
  • Proteins
  • Regulations
  • Statistical Analysis
  • Tyrosine

Fields of Study

  • Biology

Readers

  • Breast cancer cell signaling and growth regulation.