Metal Ion Complexes for Antibody Imaging and Therapy in Breast Cancer

Abstract

The goals of this project are to prepare and test novel bifunctional chelates based on DOTA to antibodies directed to CEA (carcinoembryonic antigen) and Her2/neu. The conjugates will be radiolabeled with (111)Indium for tumor imaging and (90)Yttrium for tumor therapy. In the first year of the project we have shown that the CEA positive MCF 7 cell line transfected with Her2/neu can be grown in nude mice injected with estrogen pellets and used as a tumor model. Since we have previously conjugated anti-CEA antibody T84.66 with DOTA and shown it to target CEA positive tumors, we began our work with the anti-Her2/neu antibody 4D5. First, 4D5 was shown to stain the MCF 7/Her2/neu cells (>75%).

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADA382774

Entities

People

  • John E. Shively

Tags

DTIC Thesaurus Topics

  • Albumins
  • Animals
  • Antibodies
  • Biomedical Research
  • Breast Cancer
  • Cell Line
  • Cells
  • Chemical Compounds
  • Estrogens
  • Laboratory Animals
  • Macromolecules
  • Materials
  • Molecules
  • Neoplasms
  • Polymers
  • Rodents
  • Therapy

Readers

  • Oncology (Cancer Research).