Secretory Heat Shock Protein - gp96-Ig Chaperoned Her-2/new Vaccines
Abstract
A fusion between the heat shock protein gp96 and the Fc portion of immunoglobulin IgG1 was created deleting simultaneously the endoplasmic reticulum retention signal of gp96. The product, gp96-Ig, was transfected into the lymphoma line EG7 to generate EG7-gp96-Ig. Transfected gp96-Ig is secreted by the tumor cells and can be detected by ELISA. While EG7 and mock transfected EG7 form tumors in mice, EG7-gp96-Ig is rejected. Rejection requires CD8 cells but is not dependent on CD4 cells. Rejection is unimpeded when monocyte/macrophages are inactivated by Carrageenan. Tumor-secreted gp96-Ig generates protective, tumor specific tumor immunity and CD8 dependent T cell memory. It is concluded that tumor secreted gp96-Ig results in specific activation of CD8 cells upon in vivo inoculation in mice. The molecular mechanism by which CD8 T cells are activated is under investigation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA382781
Entities
People
- Eckhard Podack
Organizations
- University of Miami