Identification of Markers of the Invasive Phenotype in Human Breast Cancer (96 Cancer)
Abstract
Our goal is to identify genes involved in the development of the invasive phenotype as these may offer predictive markers and markers of risk of invasive disease in pre-invasive lesions. We have applied our tissue based strategy to directly identify differentially expressed genes between pre-invasive in-situ (DCIS) and adjacent early invasive tumor cell populations and have completed assessment of S tumors and identified two promising candidate invasion' genes. Psoriasin (SlOO A7) has been pursued by assessment of persistent expression in invasive tumors, showing that high levels correlate with ER-ve and node +ve status. The functional role has been pursued by transfection and overexpression in invasive (MDA-MB-231) and pre-neoplastic (MCF10AT) breast cell lines, and yeast 2-hybrid assay to search for interacting proteins. Lumican is a small leucine-rich proteoglycan, overexpressed in stroma adjacent to in-situ elements and at the margin of invasive elements. Lumican has been pursued by detailed study of expression pattern in-vivo and comparison with expression profiles of related proteoglycans. The functional role is being pursued by study of transfection to achieve overexpression in fibroblasts, to assess the effect of this on epithelial cells in in-vitro assays.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1999
- Accession Number
- ADA382826
Entities
People
- Peter H. Watson
Organizations
- University of Manitoba