Expression of Brucella Antigens in Vaccinia Virus to Prevent Brucellosis in Humans: Protection Studies in Mice

Abstract

Previous and present work indicates that WRvaccinia/Brucella antigen recombinants are not conferring protective immunity. Based on our present studies and the finding that Brucella Cu/ZN SOD and L7/Ll2 proteins are protective antigens and that the presence of IL-12 is necessary at the moment of immunization, we conclude that one systemic vaccination with rvaccinia expressing 1 or 2 protective Brucella antigens with or without simultaneous production of IL-12, will not confer protective immunity. Systemic revaccination with the rvaccinia will most probably not change this situation because of immunity developed the vaccinia vector. Mucosal revaccination may overcome this problem and induce protection; such experiments are underway.

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Document Details

Document Type
Technical Report
Publication Date
Aug 01, 2000
Accession Number
ADA382850

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  • Gerhardt G. Schurig

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  • Biological Factors
  • Biomedical And Dental Materials
  • Cells
  • Chemistry
  • Diseases And Disorders
  • Genetic Code
  • Immunity
  • Immunization
  • Infection
  • Infectious Diseases
  • Lymphatic System
  • Lymphocytes
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  • Vaccination
  • Vaccines

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  • Biology
  • Medicine

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  • Immunology

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  • Biotechnology
  • Biotechnology - Cancer Biotech