Growth Suppressors of Breast Cancer Cells

Abstract

A genetic system has been established for the isolation of candidate tumor suppressor genes in MCF7 breast cancer cells. This system includes the IPTG-inducible episomal vector, pEpiLac, which combines the advantages of inducible expression systems and extrachromosomal replication systems. A cDNA library from normal human breast epithelial cells was constructed in the pEpiLac vector system, and transfected into a cell line (LAP5) derived from the human breast cancer MCF7 cells which allows the inducible expression of the library DNA. After several rounds of selection, 12 sequences that confer growth inhibitory effects of on LAP5 cells were isolated. Sequence analysis revealed that some of these sequences are fragments of known genes, while others represent novel gene sequences with no know functions.

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Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1999
Accession Number
ADA382887

Entities

People

  • Lester Lau

Organizations

  • University of Illinois at Chicago

Tags

DTIC Thesaurus Topics

  • Amino Acids
  • Biological Sciences
  • Breast Cancer
  • Calcium Compounds
  • Cancer
  • Cell Line
  • Cells
  • Chemistry
  • Dna Sequence Analysis
  • Epithelial Cells
  • Genetic Code
  • Genetic Structures
  • Indicator Dyes
  • Mammary Glands
  • Neoplasms
  • Proteins
  • Sequence Analysis

Fields of Study

  • Biology

Readers

  • Molecular Biology and Genetics
  • Molecular Genetics

Technology Areas

  • Biotechnology