MR Imaging and Gene Therapy of Breast Cancer
Abstract
The main purpose of this Career Development Award is for the P.I. to receive training in the field of Cancer Biology and Virology, so that she can apply her expertise, Magnetic Resonance Imaging, for developing non-invasive techniques to monitor gene therapy induced cellular and vascular changes in breast cancer. The P.I. has attended several courses including Virology, Cancer Biology, Molecular Biology and Molecular Biology of Cancer during the initial year of the funding period. She has also learned the protocol of encoding genes into adenovirus. A research assistant has been recruited and is currently undergoing training. They are now working on examination of tissues for various morphological and cellular characteristics. For search of appropriate recombinant viral systems that are expected to cause direct cell killing and indirect killing via microvasculature damage, the P.I. has done extensive literature search. The search results were also used in a proposal submitted to California Breast Cancer Research Program. A plasmid containing VEGF121 has been provided to the P.I. for this project. The transcfection of this gene (sense and anti-sense) into adenovirus are in progress with the support of the UCI Viral Vector Facility. The gene therapy monitoring experiments with MRI will start as soon as the virus is available.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1999
- Accession Number
- ADA382893
Entities
People
- Min-ying Su
Organizations
- University of California, Irvine